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KMID : 1005220100250020089
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Journal of Embryo Transfer
2010 Volume.25 No. 2 p.89 ~ p.92
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Offsprings Produced by Transcervically Inseminating Frozen-thawed Semen into Uterus of a Estrus-induced Saanen Goat during Non-breeding Season
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Yong Hwan-Yul
Kim Min-Ah
Bae Bok-Soo
Kim Seung-Dong
Jo Shin-Il
Lim Yang-Mook
Yoo Mi-Hyun
Ha Yong-Hee
Oh Chang-Shik
Kim Doo-Hee
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Abstract
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We report herein the successful results of estrus induction, sperm cryopreservation and kids born by transcervical insemination of frozen-thawed semen in a Saanen goat. Flugestone acetate (FGA: 60 mg) was inserted into vagina for 15 days. The goat was intramuscularly injected with 400 IU PMSG and 200 IU hCG (: Intervet, Korea) a day before withdrawal of the FGA sponge. Follicles and corpora lutea were identified on both ovaries by laparoscopy. Artificial insemination was performed 46 hours after removal of FGA sponge. The concentration of frozen-thawed semen was and 0.5 ml of frozen-thawed semen was transcervically inseminated into uterine body under anesthesia. Three kids, all females, were born 144 days after artificial insemination. This is the first report producing kids by transcervical insemination of frozen-thawed semen in a Saanen goat of which the estrus was induced by FGA sponges, PMSG and hCG during non-breeding season in Korea.
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KEYWORD
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estrus, frozen-thawed, induction, insemination, Saanen
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